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anti ebna1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti ebna1
    Anti Ebna1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ebna1/EBV+EBNA-1+Antibody/pm41731944-180-29-31
    Average 95 stars, based on 65 article reviews
    anti ebna1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Momordica anti-HIV protein MAP30 abrogates the Epstein-Barr virus nuclear antigen 1 dependent functions in host cells
    Article Snippet: .. The proteins of interest were resolved by SDS-polyacrylamide gel electrophoresis (PAGE) and identified by immunoblotting analysis using the following antibodies: DYKDDDDK tag (Abnova MAB7373; 1:1000), EBNA1 (Santa Cruz sc-81581; 1:200), Actin (Abnova MAB12828; 1:2000), EBNA2 (Millipore MABE8; 1:500), and His-tag (Merck 05–531). .. The proteins were detected and visualized using an ECL detection kit (PerkinElmer PK-NEL105).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Momordica anti-HIV protein MAP30 abrogates the Epstein-Barr virus nuclear antigen 1 dependent functions in host cells
    Article Snippet: .. The proteins of interest were resolved by SDS-polyacrylamide gel electrophoresis (PAGE) and identified by immunoblotting analysis using the following antibodies: DYKDDDDK tag (Abnova MAB7373; 1:1000), EBNA1 (Santa Cruz sc-81581; 1:200), Actin (Abnova MAB12828; 1:2000), EBNA2 (Millipore MABE8; 1:500), and His-tag (Merck 05–531). .. The proteins were detected and visualized using an ECL detection kit (PerkinElmer PK-NEL105).

    Western Blot:

    Article Title: Momordica anti-HIV protein MAP30 abrogates the Epstein-Barr virus nuclear antigen 1 dependent functions in host cells
    Article Snippet: .. The proteins of interest were resolved by SDS-polyacrylamide gel electrophoresis (PAGE) and identified by immunoblotting analysis using the following antibodies: DYKDDDDK tag (Abnova MAB7373; 1:1000), EBNA1 (Santa Cruz sc-81581; 1:200), Actin (Abnova MAB12828; 1:2000), EBNA2 (Millipore MABE8; 1:500), and His-tag (Merck 05–531). .. The proteins were detected and visualized using an ECL detection kit (PerkinElmer PK-NEL105).

    Immunostaining:

    Article Title: Epstein-Barr virus mRNA vaccine synergizes with NK cells to enhance nasopharyngeal carcinoma eradication in humanized mice
    Article Snippet: For H&E staining, sections were stained with Harris hematoxylin and 2% eosin (Servicebio, G1005). .. For immunostaining, antigen retrieval was performed and then blocked with 10% goat serum (Solarbio, SL038) for 1 h, followed by incubation with primary antibodies against CD56 (1:200, Affinity, DF7832), granzyme B (1:200, Affinity, AF0175), perforin (1:200, Affinity, DF6004), CD4 (1:100, Abcam, DF16080), CD8 (1:100, Abcam, ab17147), IFN-γ (1:100, Affinity, DF6045), and EBNA1 (1:100, Santa Cruz, 81581) at 4°C for overnight. .. Finally, sections were incubated with corresponding HRP-labeled secondary antibodies for 30 min at 37°C and visualized using DAB Chromogenic Kit (Servicebio, G1212), followed by counterstaining with hematoxylin (Servicebio, G1004).

    Article Title: Epstein-Barr virus mRNA vaccine synergizes with Natural Killer cells to enhance nasopharyngeal carcinoma eradication in humanized mice
    Article Snippet: For H&E staining, sections were stained with Harris hematoxylin and 2% eosin (Servicebio, G1005). .. For immunostaining, antigen retrieval was performed and then blocked with 10% goat serum (Solarbio, SL038) for 1 h, followed by incubation with primary antibodies against CD56 (1:200, Affinity, DF7832), granzyme B (1:200, Affinity, AF0175), perforin (1:200, Affinity, DF6004), CD4 (1:100, Abcam, DF16080), CD8 (1:100, Abcam, ab17147), IFN-γ (1:100, Affinity, DF6045), and EBNA1 (1:100, Santa Cruz, 81581) at 4◦C for overnight. .. Finally, sections were incubated with corresponding HRP-labeled secondary antibodies for 30 min at 37◦C and visualized using DAB Chromogenic Kit (Servicebio, G1212), followed by counterstaining with hematoxylin (Servicebio, G1004).

    Incubation:

    Article Title: Epstein-Barr virus mRNA vaccine synergizes with NK cells to enhance nasopharyngeal carcinoma eradication in humanized mice
    Article Snippet: For H&E staining, sections were stained with Harris hematoxylin and 2% eosin (Servicebio, G1005). .. For immunostaining, antigen retrieval was performed and then blocked with 10% goat serum (Solarbio, SL038) for 1 h, followed by incubation with primary antibodies against CD56 (1:200, Affinity, DF7832), granzyme B (1:200, Affinity, AF0175), perforin (1:200, Affinity, DF6004), CD4 (1:100, Abcam, DF16080), CD8 (1:100, Abcam, ab17147), IFN-γ (1:100, Affinity, DF6045), and EBNA1 (1:100, Santa Cruz, 81581) at 4°C for overnight. .. Finally, sections were incubated with corresponding HRP-labeled secondary antibodies for 30 min at 37°C and visualized using DAB Chromogenic Kit (Servicebio, G1212), followed by counterstaining with hematoxylin (Servicebio, G1004).

    Article Title: Epstein-Barr virus mRNA vaccine synergizes with Natural Killer cells to enhance nasopharyngeal carcinoma eradication in humanized mice
    Article Snippet: For H&E staining, sections were stained with Harris hematoxylin and 2% eosin (Servicebio, G1005). .. For immunostaining, antigen retrieval was performed and then blocked with 10% goat serum (Solarbio, SL038) for 1 h, followed by incubation with primary antibodies against CD56 (1:200, Affinity, DF7832), granzyme B (1:200, Affinity, AF0175), perforin (1:200, Affinity, DF6004), CD4 (1:100, Abcam, DF16080), CD8 (1:100, Abcam, ab17147), IFN-γ (1:100, Affinity, DF6045), and EBNA1 (1:100, Santa Cruz, 81581) at 4◦C for overnight. .. Finally, sections were incubated with corresponding HRP-labeled secondary antibodies for 30 min at 37◦C and visualized using DAB Chromogenic Kit (Servicebio, G1212), followed by counterstaining with hematoxylin (Servicebio, G1004).

    Lysis:

    Article Title: Multi-Omics Exploration of the Mechanism of Curcumol to Reduce Invasion and Metastasis of Nasopharyngeal Carcinoma by Inhibiting NCL/EBNA1-Mediated UBE2C Upregulation
    Article Snippet: .. Other solutions and antibodies used are as follows: DMEM (Gibco, Grand Island, NE, USA); FBS (Gibco, Grand Island, NE, USA); penicillin-streptomycin (Solarbio, Beijing, China); RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China); BCA protein assay kit (Beyotime Biotechnology, Shanghai, China); Antibody against β-actin (ZSGB-BIO, Beijing, China); NCL (Abcam, Cambridge, UK); EBNA1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); and UBE2C (P22620, ProMab, Richmond, CA, USA). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Multi-Omics Exploration of the Mechanism of Curcumol to Reduce Invasion and Metastasis of Nasopharyngeal Carcinoma by Inhibiting NCL/EBNA1-Mediated UBE2C Upregulation
    Article Snippet: .. Other solutions and antibodies used are as follows: DMEM (Gibco, Grand Island, NE, USA); FBS (Gibco, Grand Island, NE, USA); penicillin-streptomycin (Solarbio, Beijing, China); RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China); BCA protein assay kit (Beyotime Biotechnology, Shanghai, China); Antibody against β-actin (ZSGB-BIO, Beijing, China); NCL (Abcam, Cambridge, UK); EBNA1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); and UBE2C (P22620, ProMab, Richmond, CA, USA). ..

    Control:

    Article Title: EBV-Encoded MicroRNA-BART17-3p Targets DDX3X and Promotes EBV Infection in EBV-Associated T/Natural Killer–Cell Lymphoproliferative Diseases
    Article Snippet: Finally, the expression levels of targeted proteins were detected with a Pierce ECL Western blotting substrate system (Bio-Rad). .. Specific antibodies were employed for DDX3X (1:2000; NOVUS), Flag (1:2000; Santa Cruz Biotechnology), BZLF1 (1:500; Santa Cruz Biotechnology), LMP1 (1:200; Santa Cruz Biotechnology), and EBNA1 (1:1000; Santa Cruz Biotechnology), with β-actin (1:2000; AntGene) used as the control. ..



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